Application

Research CategoryNeuroscience

Western blot: 1-10 µg/mL using ECL. 83-90kDa bands, membrane preparations are recommended (Masilamani et al., 1999).

ELISA: 0.5-1.0 mg/mL using 1 mg/mL control peptide to coat plate.

Optimal working dilutions must be determined by the end user.

Research Sub CategoryIon Channels & TransportersNeuroinflammation & Pain

This Anti-Epithelial Sodium Channel-β Antibody is validated for use in ELISA, WB for the detection of Epithelial Sodium Channel-β.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

A 20 amino acid peptide sequence near the N-terminus of rat ENaC beta. The immunogen sequence is 100% conserved in mouse, 94% in rabbit, 81% in human, 76% in ovine and 64% in xenopus.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin in PBS containing 0.1% BSA.

Specificity

Recognizes rat Epithelial Sodium Channel beta (ENaC beta). The immunogen shows no significant sequence homology with other ENaC subunits.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.